The Lightning Project

The ongoing saga of the PNG Lightning Maroon Clownfish Breeding Project

Browsing Posts in Lighting Maroon Clownfish

Online retail saltwater and freshwater aquarium fish direct from Matt Pedersen via MiniWaters.FISH - shop online now!

Online retail saltwater and freshwater aquarium fish direct from Matt Pedersen via MiniWaters.FISH – shop online now!

Since the Lightning Project started, and particularly once progeny first became available, I’ve had countless inquiries about purchasing Lightning Maroon Clownfish direct from me.  In 2013 and 2014, all the offspring I had to offer were sold exclusively through Blue Zoo Aquatics and the team there. 2015 saw a dramatic uptick in production and availability from Sea & Reef Aquaculture; meanwhile for multiple reasons I don’t think I reared a single fish in 2015.

However, the holdback pair, MD1 and MD2, started spawning for my good friend Mike Doty, and thus, we’ve had F2 fish available as I announced late last year.  There are also very limited F1 Lighting Maroon offspring straight from the wild pair that I will make available.

A WSYIWYG F1 PNG Lightning Maroon Clownfish, 2 years old, from the original wild pair!  Available on MiniWaters.FISH

A WSYIWYG F1 PNG Lightning Maroon Clownfish, 2 years old, from the original wild pair! Available on MiniWaters.FISH

To be frank, we’ve been offering these fish at wholesale to stores, but have had very limited interest, which is surprising since so many of the shops had been asking for them for so many years.  Furthermore, our breeding here in Duluth, MN, is currently the only production of high-coverage to all-white Lightning Maroon Offspring that I’m aware of, which means the shops and retailers that have wholesale accounts with us have access to very exclusive premium quality Lightning Maroons to ultra quality Lightning Maroon Clownfish that no one else can get!  These are the fish like the pair I held back; the entire bodies of these fish are expected to break up over time into the lace-like network of red spots.  And of course, we have plenty of more traditional type standard grade Lightning Maroon Clown offspring as well, and they’re not 100% related to the Sea & Reef bloodlines either.

You can find premium grade Lightning Maroon Clownfish like this for sale at MiniWaters.FISH

You can find premium grade Lightning Maroon Clownfish like this for sale at MiniWaters.FISH

When it comes right down to it, as breeders, we need to sell fish. And as much as I run and offered wholesale exclusively for the past few years to “support the LFS”, if the stores aren’t buying what we’re producing, yet we know you guys WANT them, we had to find another way.

Yes, a solid white juvenile like this is going to be covered in pattern in a couple years - Lightning Maroons like this for now only come from Duluth MN!  Buy them at MiniWaters.FISH

Yes, a solid white juvenile like this is going to be covered in pattern in a couple years – Lightning Maroons like this for now only come from Duluth MN! Buy them at MiniWaters.FISH

So as of January 1st, 2016, I personally launched MiniWaters.FISH.  This is exclusive, direct from Matt Pedersen offerings of mostly captive-bred marine / saltwater fish (with the occasional freshwater offerings just to mix things up). Some fish are produced here, some produced by other breeders, and all carefully chosen to suit my own tastes first.  In short, I’m only going to intentionally stock fish that IF they never sell, I’ll be happy to call them pets for their rest of their lives!  If you like my tastes, then you’ll like what I’m offering.  If you want to buy Lightning Maroon Clownfish, well, for obvious reasons, this is the first place I’d suggest you look!

And I’m still offering wholesale to local shops; my retail pricing is such that any shop should be able to purchase from my wholesale list and, if they desire, undercut my online pricing while still making an industry-standard basic retail markup on livestock. See the MiniWaters.FISH wholesale page for more information – I’m STILL trying to “support the LFS”!

Customers in the upper midwest have an added bonus; residents of MN, WI, Northern IL and Northern IA can get overnight shipping for as little as $10!  Yes, MN retail customers, you get stuck with full Duluth city-rate sales tax..it is what it is…but shipping in the upper midwest is a screamin’ deal!

Standard Grade PNG Lightning Maroon Clownfish - shop for them at MiniWaters.FISH

Standard Grade PNG Lightning Maroon Clownfish – shop for them at MiniWaters.FISH

So please be sure to check out MiniWaters.FISH.  Please “like” the MiniWaters.FISH Facebook page too so you get updates.

I won’t be posting much in the way of “promoting sales” here on The Lightning Project per-say, as that’s not really the point of this website.  But, breeders need to sell their fish and aquarists need to buy them, so…wholesale or retail, drop me a line at MiniWaters.FISH and maybe I can send something your way!

There are pretty crappy images, but they are the offspring of the MD1 X MD2 Lightning X White Stripe pair.

Wholesale customers have access to these fish at this time.

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Been a while since I shared photos of the F1 spawning Lightning X Lightning pair.  So here’s how they look now:

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Surprised to hear that tonight, Mike Doty’s pair of Lightning Maroon Clownfish had thrown down eggs. This is one of the freely distributed “genetic repository” pairs I placed locally, just in case anything ever happened here at home.

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The female Lightning Maroon, MD1, and male Morse Code Maroon, MD2, represent the first F1 sibling pair that I am aware of which should replicate the pairing of their parents. We already know of the results Soren Hansen had when pairing an F1 Lightning Maroon Clownfish with a wild White Stripe Maroon; I expect that Mike will see a 50/50 White Stripe/Lightning spread in the F2 generation from Mike’s parents.

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As promised, I finally got time to look at the offspring that Mike Doty had managed to rear from my F1 Lightning X Lighting Maroon Clownfish pair.  While Mike had initially stated he felt the split was 50/50, today’s visit showed something different.

All told, I only managed to count 3 white stripe offspring in the BRT. Meanwhile, there appeared to be 6 distinctive Lightning Maroon-type offspring. What I didn’t see were any fish that, at this point, looked atypical from either known phenotype.

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With only 9 (approximate) juveniles, the sample is far from conclusive. The 3/6 split could be representative of anything from a 50/50 split to a 25/75 split. A new phenotype could be missing because none simply survived in this first successful run.

That said, the 3/6 split, if a valid sample, would represent something we perhaps don’t want to  see.  It would imply that Lightning is straight dominant, but it would also imply that a double-dose of Lightning is in fact fatal to the offspring and they fail to develop.  This is one of the current working hypotheses for the Snowflake gene in Ocellaris, and anecdotal reports continue to bolster that line of thinking (reports are anecdotal in so much as a breeder saying “It’s about a 60-70% snowflake result” is not the same as a breeder saying “I got 140 Snowflakes and 65 wild types in this clutch”).

It is fair to say that there is still hope for these fish – they are QUITE YOUNG and all we can truly discern at this point is that they outwardly either are white stripes or aberrant.  My tune could change as these babies develop, or if subsequent results are different. It is not surprising to me though, to see similarities emerge in various mutation types.  We already have Picasso/Platinum and the sister mutation of DaVinci/Wyoming White. Much as I wonder if these twin mutations could represent the same gene in different species (or simply a similar genetic mutation in sibling species), would we come to find out that Lightning is in fact not unlike Snowflake? After all, genetic analysis has revealed that the Maroon Clownfish are in fact very closely related to the Percula/Ocellaris complex, so it would not be surprising to find similar or the same genes present in all these species based on their common shared ancestor.

It is also an interesting footnote to observe that these tiny juvenile clownfishes DO represent a the first F2 generation of Lightning Maroons.

Here’s a quick rundown.

The 5th spawn of the F1 Lightning X Lightning Maroon Clownfish pairing was collected and hatched by Mike Doty while I was away.  In short, he scraped off the eggs, hatched them in a 1 gallon jar in a water bath with simple aeration, 75% clean new water.  Come November 28th, Mike relayed that settlement had started.  The moment we’ve been waiting for was here – is there something new?

Well, Mike’s first words were “About 90% sure we have some normal striped fish.”

This, of course, does the following:

  • likely rules out Lightning as a simple recessive gene. If it WAS recessive, then both parents would be “double dose” aka. homozygous, represented as l/l, which means that each parent could only contribute a recessive lightning gene, and thus, each offspring would also get one copy each, one from mom, one from dad, and thus, could only be l/l as well. For the moment, while another couple test matings will bolster the data, the fact that there are white-stripe offspring pretty much precludes this being a standard single allele, single locus, recessive trait.
  • does not rule out straight dominance. If it was straight dominance, and each parent is “single dose” aka. heterozygous, represented as L/+, then 25% of the offspring would not get a gene from either parent, and thus, 25% would be white stripe maroon clownfish.
  • nor does it rule out partial dominance. This of course, would work the same way as dominance, except that 25% of the offspring would get a lightning gene from EACH parent, and would be homozygous for Lightning, represented as L/L. This is the scenario that most people are hoping for, because with the new homozygous state, there comes the potential for a new phenotype that could be different from the Lightning that we know.

As of today, 12-8-2014, I spoke with Mike briefly and have to relay this news – while he doesn’t have many babies left, he believes that the phenotype split is roughly 50/50.  That is to say, half white stripes, half lightnings.  So far, he also has not seen anything unique or new in this F2 generation.  I have yet to see the babies for myself, and have yet to take pictures or do a headcount, but these cursory, informal results, mirror another clownfish mutation that seems to not fit the mold as we’d expect – SNOWFLAKE in Ocellaris.  It’s my hope to get over to Mike’s today yet to see for myself.

In other news, the 6th spawn of the F1 Lightning X Lightning pair was put down on 11/29/2014. It appears I finally won the battle of the tiles:

LxL Spawn #6

LxL Spawn #6

Meanwhile, I brought some new clownfish into the fishroom earlier in November (the 19th and 22nd) and was trying out the new Ick-Shield food from New Life Spectrum.  This is basically a Chloroquin-laced pellet food that is meant primarily to prevent disease such as Crytopcaryon, Amyloodium, Brooklynella etc…pretty much the things which are sensitive to the active ingredient. I decided to not simply feed this fish to the new arrivals, but also to feed it to one of my holding systems AND the wild Lightning Maroon and her mate as preventative medication, just in case.

Well, it turns out that there is an unfortunate side effect to this feed; it seems to shut down breeding activity.  All my routine pairs stopped spawning. The Lightning and her mate did finally put down a spawn on December 1st, 2014, #46.

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Unfortunately, it appears as though the spawn was not fertilized…the eggs didn’t develop, and after 48 hours they were gone. A few days after that, I read, anecdotally, that Chloroquin can cause male sterility??? Not permanent according to the rumor, but certainly a potential setback. As far as the efficacy of the food, my jury is out. The larger fish which were feeding well on it by and large remained disease free, but not all did.  I still had a Brooklynella outbreak, although not in the fish I would have necessarily expected. Once that outbreak started, it then affected other fish as well despite their feeding on the pellets.  I’m also seeing either Cryptocaryon or Amyloodium on fish which were visually “clean” upon arrival, which were in dedicated QT systems, feeding on this food from day one.  So the question here is were they simply getting ENOUGH feed as they were small fish which cannot readily eat the small pellet size.

So of course, one is left with questions, not answers.  There is no way to say the food didn’t work, nor is there any way to prove that it does work. Absence of disease is not proof of prevention, that much I know for certain. Lack of a cure, or lack of prevention, which IS documented, only raises questions about why it didn’t work as suggested and certainly requires investigation (eg. would a smaller pellet size be better accepted…could these failures stem from simply lack of feeding, or lack of sufficient feeding, thus insufficient dose to the fish?).

Circling back to LxL Spawn #6, as the week progressed an interesting change in behavior occurred starting around December 4th, 5 days post spawn.  The larger female F1 Lightning became belligerent towards the male, and over the day drove him from nest tending duties.  December 5th, a Friday, would have been 6 days post spawn, and the night of the first hatching.  I was simply swamped with preparations for sending our dog to live with my brother, and failed to pull the tile.  By morning, Saturday, December 6th, 2014, it appeared that I had not missed much…most if not all the eggs were still there. The pair remained at odds.  We left for the weekend to ship our family dog, and upon Sunday, December 7th, there were still a few dozen eggs remaining, although they appeared potentially dead and disappeared throughout the day.  By nightfall, the pair was starting to be less antagonistic, but I am still keeping a close eye on them.  Hopefully, we’ll get another spawn soon – this was the only mature pair in the house that didn’t receive Chloroquin-laced foods (as they don’t reside in the fishroom with the rest of the fish).

It’s the 7th night for Spawn #43 which was spawned on 10-22; we’re on the night of 10-29 going into 10-30 now; there wasn’t any noticeable hatch on the tile over the 6th night (I left it with the parents yesterady)….it will get pulled.  This is what Spawn #43 looked like when it was laid fresh on 10-22…I should also point out this is the first time I’ve taken pictures of the F0 original wild pair of Lightning Maroon X White Stripe PNG Maroon Clownfish.

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The larvae from spawn #42 that are still alive have largely settled out…it was a small group, but I’ll find a way to work with them.  More importantly, it looks like LM X LM Spawn #2 might be over…I have not found any larvae in the BRT, so one more look, I repurposed  it for Spawn #43.  The eggs got the usual dip in H2O2, but started hatching during it, so they were moved straight into the BRT with 8 gallons of broodstock water and a few ML of RotiGrow Plus (only thing I had thawed…it’ll be fine for the first 24 hours and by that point I’ll have RotiGreen Omega up and running).

The most frustrating news is arguably LM X LM Spawn #3.  So..to recap.  Here was the first spawn:

F1 (Lightning X Lightning) first spawn for the Lightning Maroon Clownfish

 

So…I took some proactive steps and tiled the back wall of the tank.

This was LM X LM (or LxL if you prefer) Spawn #2 on the first attempted hatch night:

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Yeah..that gray patch on the side..those are the eggs…

So that didn’t turn out obviously…so I tiled the side wall as well.

Here is Spawn #3, laid on 10/27/2014:

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The internet shorthand FML seems somehow appropriate. Clearly the pair is flipping me the middle fin.

 

So yeah…October 2nd, I’m just walking by the tank and I see this…

F1 (Lightning X Lightning) first spawn for the Lightning Maroon Clownfish

We are about to embark on the next exciting chapter of the Lightning Maroon Clownfish and genes.  My hypothesis is that the Lightning gene is partially dominant or dominant. The offspring from this spawn, if successfully raised, will yield important answers that may shed new light on the genetics at play. Will we see something NEW come out of this? Well..I certainly hope so and it looks like I’ll get to name it!

Let’s give that a closer look:

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The Lightning Maroon Clownfish at the Great Lakes Aquarium enjoy a brief feature on the homepage of the Duluth News Tribune on the morning of Sept. 7, 2014

The Lightning Maroon Clownfish at the Great Lakes Aquarium enjoy a brief feature on the homepage of the Duluth News Tribune on the morning of Sept. 7, 2014

The Lightning Maroon Clownfish offspring (GL1 and GL2) I donated to the Great Lakes Aquarium in 2013, are now officially on display.

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GL1, the lower fish, foreground, carries the Lightning gene; it’s whit striped mate, above, does not.

Late on Saturday evening, one of my FISHING friends (not to be confused with FISH friends) posted on Facebook that the official story had run online; this Saturday, September 7th, 2014, the Duluth News Tribune is running a story by Alysee Shelton about the Lightning Maroon Clownfish and its mate, which are now on display at the Great Lakes Aquarium here in Duluth, MN. You can ready the story online here for the next coupld days - http://www.duluthnewstribune.com/content/nemo-great-lakes-aquarium-duluth-fish-breeder-shares-lightning-maroon-clownfish-public - Now, I should point out that the article isn’t 100% accurate. As you all know, I like to indulge in a bit of mythbusting (aka. Fact Correcting) here at the Lightning Project…the Duluth News Tribune will “suffer my wrath” (meant very tongue in cheek, affectionately), receiving a healthy dose of disambiguation just like everyone else.

For starters, the fish were actually delivered to the GLA on August 2nd, 2013, which means they were behind the scenes for just over a year before being put on display, not the “6 months” the article mentions. The year behind the scenes is perfectly understandable given that aquarists were VERY busy with the new “Shipwrecks Alive” exhibit that was being put in place in the rotating exhibit hall to replace the “Masters of Disguise” exhibit, which was the replacement for the “Seahorses” exhibits I never got to see…but there’s another reason which I’ll cover in a second.

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I would remind readers that while this article suggests that “[SEASMART] decided to send that fish to Matt Pedersen, an international marine aquarium fish breeder in Duluth.”, that it was actually a) Blue Zoo Aquatics who had the final say in whether I would receive the fish (although my understanding is that all parties felt it was a good move) and b) they didn’t just “send” me the fish…yes, the rumors still persist that I was just “given” the Lightning Maroon!

Jay Walker is a great guy and Operations Manager at the aquarium; I LOVE that QUARANTINE, a practice that too many aquarists skimp on, was mentioned at great lengths in this media coverage and attributed to Walker.  To pull a shortened version of Walker’s comments, “quarantine lasts for a minimum of 30 days, and within this time we do prophylactic treatments for diseases, developing husbandry practices and observations. This process can last for more than 30 days depending on the situation.”  I wish to just elaborate here, that in the case of the Lighting Maroon Clownfish and its normally patterned mate, a BIG part of the actually year-long “quarantine” was the process of size-differentiation leading into successful pairing (“Mating” as the Duluth News Tribune called it in reference to when I first paired the wild fish here in my dining room…and no, not pairing with wine…).  Yes, the GLA didn’t try to accomplish pairing these sibling fish in just a month or two…

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Shelton’s article also glosses over the actuality of numbers a bit, but that’s more due to the brevity of the piece and a word we’re all forced to deal with.  For example, “produced their offspring, classified as lightning maroon clownfish, on June 29, 2012.” As most Lightning Project followers already now, only about 50% of the offspring wind up displaying the mutated phenotype (appearance) and therefore, only about half of them are actually “Lightning” Maroon Clownfish.  I should mention this because one of my worst fears is that someone picks up this story and starts talking about the fish as if they are a NEW SPECIES (which they are certainly NOT). I will also point out a subtle fact that cannot be discerned from the article as written – the GLA only has one Lightning Maroon Clownfish; it is paired with a White Stripe (Normal) sibling. When you get caught up using a word like fish (same when singular or plural) I realized, as I read this piece, that you never can really tell whether you are talking about one, two, or for that matter dozens/hundreds/thousands.

The article went on to state that “Great Lake Aquarium officials believe they are the first aquarium in the world to have these fish.” – I’ll emphatically say that “believe” suggests perhaps more room for doubt than is actually there. When it comes to “firsts” – and for a small public aquarium like the Great Lakes Aquarium, firsts DO matter – I am reasonably confident that they were the first public aquarium to have possession of a Lightning Maroon Clownfish (I could go back and check my records just to be 100% sure). Regardless of that, to the best of my knowledge, I am very confident that they can rightfully claim to have been the first and (to date) only public aquarium in the world to have this unique form on public display. I can say this with reasonable certainty given that I know where all the Lightning Maroons in my fishroom have gone…so unless someone “changed their minds”, I would presume that the Lightning Maroons that were sold in 2013 and 2014 are still in the same breeding programs they were when originally purchased…and not on public display.  Will other aquariums in the future display a Lightning Maroon Clownfish? Perhaps…but I am proud that the GLA stands apart in the world, at least for now.

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I am truly delighted that the fish are on display, in the capable hands of the aquarists at the GLA. And yes, anyone who feels the current retail pricing is just too much to spend on a fish, you can now see one for the price of admission at a unique public aquarium on the shores of St. Louis Bay on Lake Superior. My special thanks for Alysee Shelton for taking the time to craft her article for the Duluth News Tribune – in a few hours I hope to see it in print!

The first second-generation Lightning Maroon Clownfish, progeny of a F1 Lightning Maroon mated to an unrelated, Wild (F0) PNG White Stripe, produced by Sea & Reef Aquaculture.

The first second-generation Lightning Maroon Clownfish, progeny of a F1 Lightning Maroon mated to an unrelated, Wild (F0) PNG White Stripe, produced by Sea & Reef Aquaculture.

I had to sit on this news since last month as this was an exclusive for CORAL Magazine; now that the magazine is out I can shout it out – Sea & Reef Aquaculture has succeeded in producing a second generation of Lightning Maroon Clownfish by using LM12 (an F1 from my pair here) with an unrelated wild (FO) PNG White Stripe Maroon. Highlights include the same 50/50 offspring split, as well as the interesting fact that Sea & Reef used the Lightning as a male vs. female.

As it’s a CORAL Magazine exclusive, I invite all Lightning Maroon Clownfish fans to head over to Reef2Rainforest.com and read the EXPANDED online version there.  I should note, I actually didn’t want to write the story (I prefer to have people tell their own stories) but in the end, the job fell to me. I hope you enjoy!

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