The Lightning Project

The ongoing saga of the PNG Lightning Maroon Clownfish Breeding Project

Browsing Posts tagged H2O2

It’s the 7th night for Spawn #43 which was spawned on 10-22; we’re on the night of 10-29 going into 10-30 now; there wasn’t any noticeable hatch on the tile over the 6th night (I left it with the parents yesterady)….it will get pulled.  This is what Spawn #43 looked like when it was laid fresh on 10-22…I should also point out this is the first time I’ve taken pictures of the F0 original wild pair of Lightning Maroon X White Stripe PNG Maroon Clownfish.

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The larvae from spawn #42 that are still alive have largely settled out…it was a small group, but I’ll find a way to work with them.  More importantly, it looks like LM X LM Spawn #2 might be over…I have not found any larvae in the BRT, so one more look, I repurposed  it for Spawn #43.  The eggs got the usual dip in H2O2, but started hatching during it, so they were moved straight into the BRT with 8 gallons of broodstock water and a few ML of RotiGrow Plus (only thing I had thawed…it’ll be fine for the first 24 hours and by that point I’ll have RotiGreen Omega up and running).

The most frustrating news is arguably LM X LM Spawn #3.  So..to recap.  Here was the first spawn:

F1 (Lightning X Lightning) first spawn for the Lightning Maroon Clownfish

 

So…I took some proactive steps and tiled the back wall of the tank.

This was LM X LM (or LxL if you prefer) Spawn #2 on the first attempted hatch night:

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Yeah..that gray patch on the side..those are the eggs…

So that didn’t turn out obviously…so I tiled the side wall as well.

Here is Spawn #3, laid on 10/27/2014:

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The internet shorthand FML seems somehow appropriate. Clearly the pair is flipping me the middle fin.

 

Lots of little updates

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Spawn #41 was left to go for itself because the Lightning X Lighting Spawn #1 took priority.  Unfortunately, by the time the eggs reached the anticipated hatch night, Wed, Oct 8th, there were only 7 eyed up eggs left.  I attempted to harvest them by using a rigid air tube attached to a flexible tube to scrape and siphon them out…this broke one egg but the rest went through intact.  By the next morning in the incubation cup (a half gallon specimen cup with broodstock water), the eggs were dead.

So back to the F0 Lightning X White Stripe pair; Oct. 11 they laid Spawn #42, and tonight, Friday, October 17th, 2014, I pulled the eggs and gave them the typical H202 Bath..about 11 minutes in, we (Paul Whitby of HighDefCorals is in town to speak at our club) noticed that larvae had begun to hatch, so the dip was ended and I placed the tile in the cleaned, readied 6 gallon BRT which had 5 gallons broodstock water and 1 gallon new water.

In other news, the Lightning X Lightning pair put down their second spawn somewhere between 5 PM on Thursday, Oct 16th and 4:30 PM Friday, Oct. 17th. It’s a much larger nest, which was expected. Despite having basically tiled the entire backside of the aquarium, the pair thwarted my efforts by spawning around the corner in a tight area of black plastic background. I don’t know when the proper “harvest” date will be for these fish; I’ll like try my Vossen Larval Snagger this time around. But I also know that I’ll be putting even MORE tile into their tank now ;)

So I discovered a trick last night – if I run my finger over a clownfish nest, the fish inside the eggs will wiggle if alive. So despite having had no appreciable hatch all day, I pulled the tile, sanitized it again with H2O2, and threw it back in the larval tank.  I DID get a few eggs to hatch during the vigorous aeration in the sanitizing dip, so that further told me I was on the right path.

I didn’t get to check the nest until 4 PM today, but sure enough, there were significantly more larvae in the tank.  It required a new strong feeding with rotifers.  I once again tested the eggs…still viable, so I put some ChloramX in the water, and left the tile back in there.  They may not get checked again until tomorrow…

It’s the night of 5/31 going into 6/1/2014, and right before 2:00 AM (6/1) I pulled the tile for the sanitizing H2O2 (Hydrogen Peroxide) dip. While going through the 15 minute treatment at 4 ML per 0.5 gallons of broodstock water, I wiped out the BRT which was used for Spawn #32 and refilled it with 10 gallons of water from the broodstock tank.  When I went to check on the eggs, with 25 seconds left, I discovered that about 30 larvae had already hatched.

I moved the tile into the BRT with good air flow using a coarse air stone and turned out the lights.  I then spent a little time pipetting some of the larvae from the specimen cup, but I ultimately did not get them all and some had to go down the drain.  I had to wonder – would these larvae be viable long term, or would their exposure to H2O2 burn them up and render them useless.  So I wasn’t overly concerned, and I also didn’t want to add too much of that bath water into the BRT.

I’ll try to check on the hatch again in the next 30-45 minutes…I wonder if they’re going to go really fast, or if it’s in spurts (you may recall my concern over “morning” hatches…)

UPDATE – as of 4:20 AM, a last check of the night after just under 2 hours reveals no further large hatching occurred.  I wonder if they’ll hatch come daylight?  Hard to say…

Based on my calculations, 5/20 is the night we’re due to first have hatches. Having moved all the fry from Spawn #30 into a BRT “on the system”, I wiped down and drained their BRT, and refilled it with water from the Lightning Maroon’s broodstock aquarium. The tile with eggs (huge nest, 4″ X 2″) was placed in a 0.5 gallon specimen cup with 4 ml H2O2 for approximately 15 minutes, after which it was set up with a coarse airstone for hatching in the BRT. I confirmed that the air is flowing directly over the eggs at a moderate clip.  This *should* be a good hatch if I’ve done things right.

Side note on spawn #30 – ultimately the split was a nice dice roll – 8 Lightnings and only 3 White Stripes.

Hatch night for Spawn #15?

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Per my last post, the start of the week saw the spawning of the 15th batch, although I wasn’t around to see it.  So I’m having to guess on the spawn date.  I *thought* about pulling the eggs on Saturday night; this would have been 6 days (144 hours) post spawn, had they been laid on Monday.

I looked hard at the eggs, and decided they just didn’t look “silvery” enough for me to pull them. So I wanted 24 hours, knowing that in the past delaying when I thought things might be ready had caused the loss of a batch. However, this time the wait paid off…there were still the same number of eggs tonight, Sunday.  So we are now either 144 housr, or 170 hours post spawn.  Eggs were pulled and cleaned with roughly 3ML H2O2 (hydrogen peroxide) in a 1/2 gallon specimen container for 12+ minutes, and then into the BRT for hatching (the 10 gallon still has the near metamorphosis larvae from Spawn #14).  I saw one baby hatch IN the H2O2 dip, so it’s very possible that I gut-timed this one right.  Plenty of rotifers on hand, this could be a good run.

Tonight is the anticipated hatch night for spawn #14.  It’s a smaller clutch…probably only a few hundred eggs to hatch, and it might have partially hatched earlier in the day or even last night.  I set them up in the 10 gallon tank with blacked out sides, using 50% new water and 50% broodstock water.  The eggs were sanitized with hydrogen peroxide at a rate of 3 ml / 0.5 gallon for 10 minutes, after which they were removed and placed in the tank for hatching.  Lights on the broodstock system currently go out at 11:30 PM, but the room doesn’t reach its darkest point until 2 AM.

On an interesting side note, I may have discovered a contributing factor, if not the outright cuplrit, of the loss of all juveniles in spawn #12.  It turns out that the Ehiem heater in the tank was no longer sealed…I noticed it wasn’t working when I sanitized and cleaned the tank earlier this evening, and upon closer inspection there was moisture inside and the coils showed white discoloration (reaction with saltwater presumably).  Did this have an negative impact on the babies?  Possibly…at minimum it cold explain why metamorphosis appeared to take so long…the tank may have been running cooler (eg. 74-76) than I had wanted, and that delayed meta could explain why the babies failed to survive post meta.  Or not; it’s only one of many possible factors at play here.

So the tank has a new heater, and hopefully we get a good hatch tonight.

Spawn #13 Pulled – oops…

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So I pulled spawn #13 tonight…1 AM on 9-30-2013….since #13 was spawned on 9/24/2013, I really should have pulled on the night of 9/30/2013…which would be Monday night, not the wee-early morning hours of Monday as I type this.

What will this do?  Well, my other scatterbrained move was to once again apply a hydrogen peroxide (H2O2) bath…roughly 7.5 ML to 0.5 gallons…which is fine…’cept I ran it for 25 minutes instead of 15.  The eggs outwardly looked fine, but did I just kill them?  I hope not, and probably not.

Given that the last clutch was fine following treatment to hold for a day in artificial incubation, this gives me hope that if these are due to hatch in the next 24 hours, it will be just fine.  What I’m going to do now however, is add H2O2 at a low level (roughly 1 ML per gallon) to help keep the BRT I placed them into relatively clean (1 ML per gallon is a standard rate for incubation of Pterophyllum scalare, FW Angelfish, eggs…when dosed every 12-24 hours). This should cause no harm to the eggs but should also keep any pathogenic bacterial and such at bay.

- UPDATE - 

So….I’m adding drops of H2O2 to the water…I make it to 80 drops, roughly 4 ML and that’s when I noticed two newly hatched larvae swimming around the BRT.  So I stopped adding H2O2 and turned the light off…are these fish really going to hatch in 5 days? 2 DID.

I’m guessing it’s going to be a full and strong hatch Monday Night / Tuesday AM, provided I didn’t screw things up.

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