The Lightning Project

The ongoing saga of the PNG Lightning Maroon Clownfish Breeding Project

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White Stripe Spawn #3 seems to have hatched earlier than expected.  I was looking at the tank on Saturday, 9-13, which would’ve been the day to pull the tile, but the eggs were already gone.  So they may well be on a 5 day schedule?!

Meanwhile, Lightning Maroon Spawn #40 was laid on 9-10-2014.  I’ll be out of town late this week, which means I won’t be able to properly tend to baby lightning maroons.  So this batch, like the last, will be left to hatch and move one.  I believe I’ll be in a good position to deal with Spawn #41 if the fish are back on schedule.

That’s all.

I skipped worrying about the hatch on the last spawn…we simply have too much going on in our lives at the moment to effectively start rearing a batch of clownfish this week.  Meanwhile, on 9/7/2014, the White Stripe Maroons, the unrelated F0 pairing, put down another nest, but small.  I’ve yet to see a really large nest out of these guys, and their spawning is not consistent yet.  I will try to raise this batch so I have outcross material…and down the line I may finally opt to split this pair up to make three foundation lines of Lightnings in my fishroom.

That’s all!

So I discovered a trick last night – if I run my finger over a clownfish nest, the fish inside the eggs will wiggle if alive. So despite having had no appreciable hatch all day, I pulled the tile, sanitized it again with H2O2, and threw it back in the larval tank.  I DID get a few eggs to hatch during the vigorous aeration in the sanitizing dip, so that further told me I was on the right path.

I didn’t get to check the nest until 4 PM today, but sure enough, there were significantly more larvae in the tank.  It required a new strong feeding with rotifers.  I once again tested the eggs…still viable, so I put some ChloramX in the water, and left the tile back in there.  They may not get checked again until tomorrow…

Lots going on in the basement.

Spawn #33 is almost all through meta at this point, so this afternoon I turned on the system water.  It’s sad that I’ve never used my larviculture system as it was meant to be used by design, but hey, finally, I’m doing it right.  The system water is dripping through at a rate of roughly 1-2 drops per second…a very slow flow at this point but it should slowly flush out larviculture water over the next day or two.

Spawn #34, well, I botched it.  I did have more hatch out during the day today, but I discovered tonight that I had neglected to return the heater to the tank after I cleaned it last night. So basically the eggs went from 84F down to room temp, which was probably 76F.  Historically, any time temps go down on eggs, I’ve found it to be a bad thing.  I returned the heater to the tank, made sure it was really greened up, added some rotifers, and will now wait to see if anything else hatches out in the next 24 hours…

As of 11 AM, it’s a small hatch on Spawn #34.  I added a little RotiGreen Omega to help keep the fish off the walls, and will check a bit later to see if the hatch has improved during the day.

Spawn #33 – Starting midday on 6/9, the first larvae started showing signs of metamorphosis and settlement; white spots on the heads of the Lightnings are among the first signs; most are through meta today.

Spawn #34 – Tonight is the scheduled hatch night; I used the 4 ml H2O2 / 0.5 gallon broodstock water as a 15 minute bath before placing them in a 10 gallon; 5 gallons of broodstock water and a coarse air feed going over the nest; they’ll sit for the night and I’ll be sure to check them in the AM.  Will there be a morning hatch yet again?

It is now a fact – big Lightning Maroon Clownfish hatches are happening in the morning. I went to bed sometime after 4:30 AM on June 1st, and a few hours later, around 8:30, the kids woke me up. So, I went down to look at the BRT – just a handful of larvae.  I checked with a light, none on the bottom…just a very weak hatch.

I greened up the tub with RotiGreen Omega and added 2 gallon’s worth of rotifers from a culture. I turned on the light, left the tile in place.  Around 10:30 AM, I went down again and I now had hundreds of larvae in the BRT.

I pulled the tile and gave it back to the parents…can’t remember if that was an idea raised here, or on Facebook, but it seemed like a good way to go.  I have yet to go back down tonight, but I’ll presumably set up yet another new tank for a hatch tonight and see if we get two solid night’s worth of larvae off this batch.  Things could get interesting.

It’s the night of 5/31 going into 6/1/2014, and right before 2:00 AM (6/1) I pulled the tile for the sanitizing H2O2 (Hydrogen Peroxide) dip. While going through the 15 minute treatment at 4 ML per 0.5 gallons of broodstock water, I wiped out the BRT which was used for Spawn #32 and refilled it with 10 gallons of water from the broodstock tank.  When I went to check on the eggs, with 25 seconds left, I discovered that about 30 larvae had already hatched.

I moved the tile into the BRT with good air flow using a coarse air stone and turned out the lights.  I then spent a little time pipetting some of the larvae from the specimen cup, but I ultimately did not get them all and some had to go down the drain.  I had to wonder – would these larvae be viable long term, or would their exposure to H2O2 burn them up and render them useless.  So I wasn’t overly concerned, and I also didn’t want to add too much of that bath water into the BRT.

I’ll try to check on the hatch again in the next 30-45 minutes…I wonder if they’re going to go really fast, or if it’s in spurts (you may recall my concern over “morning” hatches…)

UPDATE – as of 4:20 AM, a last check of the night after just under 2 hours reveals no further large hatching occurred.  I wonder if they’ll hatch come daylight?  Hard to say…

Based on my calculations, 5/20 is the night we’re due to first have hatches. Having moved all the fry from Spawn #30 into a BRT “on the system”, I wiped down and drained their BRT, and refilled it with water from the Lightning Maroon’s broodstock aquarium. The tile with eggs (huge nest, 4″ X 2″) was placed in a 0.5 gallon specimen cup with 4 ml H2O2 for approximately 15 minutes, after which it was set up with a coarse airstone for hatching in the BRT. I confirmed that the air is flowing directly over the eggs at a moderate clip.  This *should* be a good hatch if I’ve done things right.

Side note on spawn #30 – ultimately the split was a nice dice roll – 8 Lightnings and only 3 White Stripes.

There are still maybe 2 larvae from Spawn #30 that haven’t settled.  The dice roll so far seems to be heavy towards Lightning offspring…but all told there are probably 10 fish or so that made it.

Spawn #31 simply didn’t work out.  The timers on the filtration worked flawlessly, but for some reason the eggs simply never hatched.  After a very small hatch on the 6th night, the eggs remained and nothing hatched on the 7th, and after the 8th night they still were there, and vanished at some point during the day.  I’m presuming they had died, not hatched.  I ultimately didn’t bother to start a run…clearly my problems with hatching are not necessarily due to my artificial incubation methodologies, but something more fundamental.

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