The Lightning Project

The ongoing saga of the PNG Lightning Maroon Clownfish Breeding Project

Browsing Posts tagged Hydrogen Peroxide

So I discovered a trick last night – if I run my finger over a clownfish nest, the fish inside the eggs will wiggle if alive. So despite having had no appreciable hatch all day, I pulled the tile, sanitized it again with H2O2, and threw it back in the larval tank.  I DID get a few eggs to hatch during the vigorous aeration in the sanitizing dip, so that further told me I was on the right path.

I didn’t get to check the nest until 4 PM today, but sure enough, there were significantly more larvae in the tank.  It required a new strong feeding with rotifers.  I once again tested the eggs…still viable, so I put some ChloramX in the water, and left the tile back in there.  They may not get checked again until tomorrow…

It’s the night of 5/31 going into 6/1/2014, and right before 2:00 AM (6/1) I pulled the tile for the sanitizing H2O2 (Hydrogen Peroxide) dip. While going through the 15 minute treatment at 4 ML per 0.5 gallons of broodstock water, I wiped out the BRT which was used for Spawn #32 and refilled it with 10 gallons of water from the broodstock tank.  When I went to check on the eggs, with 25 seconds left, I discovered that about 30 larvae had already hatched.

I moved the tile into the BRT with good air flow using a coarse air stone and turned out the lights.  I then spent a little time pipetting some of the larvae from the specimen cup, but I ultimately did not get them all and some had to go down the drain.  I had to wonder – would these larvae be viable long term, or would their exposure to H2O2 burn them up and render them useless.  So I wasn’t overly concerned, and I also didn’t want to add too much of that bath water into the BRT.

I’ll try to check on the hatch again in the next 30-45 minutes…I wonder if they’re going to go really fast, or if it’s in spurts (you may recall my concern over “morning” hatches…)

UPDATE – as of 4:20 AM, a last check of the night after just under 2 hours reveals no further large hatching occurred.  I wonder if they’ll hatch come daylight?  Hard to say…

Based on my calculations, 5/20 is the night we’re due to first have hatches. Having moved all the fry from Spawn #30 into a BRT “on the system”, I wiped down and drained their BRT, and refilled it with water from the Lightning Maroon’s broodstock aquarium. The tile with eggs (huge nest, 4″ X 2″) was placed in a 0.5 gallon specimen cup with 4 ml H2O2 for approximately 15 minutes, after which it was set up with a coarse airstone for hatching in the BRT. I confirmed that the air is flowing directly over the eggs at a moderate clip.  This *should* be a good hatch if I’ve done things right.

Side note on spawn #30 – ultimately the split was a nice dice roll – 8 Lightnings and only 3 White Stripes.

So Mike Doty has had comparable luck to some of my better “early runs”; Spawn #27 remains at his house and in a startling role reversal, I’m now watching HIS fishroom.  As of yesterday, I discovered the first post-meta offspring, a Lightning juvenile, in the warmer, more lightly-stocked right BRT.  The rest are still pre-meta fish.

Spawn #28 was pulled too early, and I should have listened more to Mike Hoang’s advice to do an H2O2 dip on eggs that don’t hatch in the first night.  It seems that the eggs remained viable on the tile up until yesterday afternoon, 4-8, but the majority never hatched. Interestingly, it wasn’t until the morning of 4-8 that the last of the eggs in with the parents had disappeared. There are a scant few larvae in the 10 gallon tank from this batch as a result.

The other news to report is that on the afternoon of 4-8-2014, the 29th spawn was put down by the Lightning Maroons.  Based on my dates, I should pull the tile for hatching next Monday, the 14th.

Hatch night for Spawn #15?

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Per my last post, the start of the week saw the spawning of the 15th batch, although I wasn’t around to see it.  So I’m having to guess on the spawn date.  I *thought* about pulling the eggs on Saturday night; this would have been 6 days (144 hours) post spawn, had they been laid on Monday.

I looked hard at the eggs, and decided they just didn’t look “silvery” enough for me to pull them. So I wanted 24 hours, knowing that in the past delaying when I thought things might be ready had caused the loss of a batch. However, this time the wait paid off…there were still the same number of eggs tonight, Sunday.  So we are now either 144 housr, or 170 hours post spawn.  Eggs were pulled and cleaned with roughly 3ML H2O2 (hydrogen peroxide) in a 1/2 gallon specimen container for 12+ minutes, and then into the BRT for hatching (the 10 gallon still has the near metamorphosis larvae from Spawn #14).  I saw one baby hatch IN the H2O2 dip, so it’s very possible that I gut-timed this one right.  Plenty of rotifers on hand, this could be a good run.

Tonight is the anticipated hatch night for spawn #14.  It’s a smaller clutch…probably only a few hundred eggs to hatch, and it might have partially hatched earlier in the day or even last night.  I set them up in the 10 gallon tank with blacked out sides, using 50% new water and 50% broodstock water.  The eggs were sanitized with hydrogen peroxide at a rate of 3 ml / 0.5 gallon for 10 minutes, after which they were removed and placed in the tank for hatching.  Lights on the broodstock system currently go out at 11:30 PM, but the room doesn’t reach its darkest point until 2 AM.

On an interesting side note, I may have discovered a contributing factor, if not the outright cuplrit, of the loss of all juveniles in spawn #12.  It turns out that the Ehiem heater in the tank was no longer sealed…I noticed it wasn’t working when I sanitized and cleaned the tank earlier this evening, and upon closer inspection there was moisture inside and the coils showed white discoloration (reaction with saltwater presumably).  Did this have an negative impact on the babies?  Possibly…at minimum it cold explain why metamorphosis appeared to take so long…the tank may have been running cooler (eg. 74-76) than I had wanted, and that delayed meta could explain why the babies failed to survive post meta.  Or not; it’s only one of many possible factors at play here.

So the tank has a new heater, and hopefully we get a good hatch tonight.

Spawn #13 Pulled – oops…

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So I pulled spawn #13 tonight…1 AM on 9-30-2013….since #13 was spawned on 9/24/2013, I really should have pulled on the night of 9/30/2013…which would be Monday night, not the wee-early morning hours of Monday as I type this.

What will this do?  Well, my other scatterbrained move was to once again apply a hydrogen peroxide (H2O2) bath…roughly 7.5 ML to 0.5 gallons…which is fine…’cept I ran it for 25 minutes instead of 15.  The eggs outwardly looked fine, but did I just kill them?  I hope not, and probably not.

Given that the last clutch was fine following treatment to hold for a day in artificial incubation, this gives me hope that if these are due to hatch in the next 24 hours, it will be just fine.  What I’m going to do now however, is add H2O2 at a low level (roughly 1 ML per gallon) to help keep the BRT I placed them into relatively clean (1 ML per gallon is a standard rate for incubation of Pterophyllum scalare, FW Angelfish, eggs…when dosed every 12-24 hours). This should cause no harm to the eggs but should also keep any pathogenic bacterial and such at bay.

- UPDATE - 

So….I’m adding drops of H2O2 to the water…I make it to 80 drops, roughly 4 ML and that’s when I noticed two newly hatched larvae swimming around the BRT.  So I stopped adding H2O2 and turned the light off…are these fish really going to hatch in 5 days? 2 DID.

I’m guessing it’s going to be a full and strong hatch Monday Night / Tuesday AM, provided I didn’t screw things up.

Spawn #12 still holding…

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Despite all the crazy effort last night to prep for a hatch on spawn #12, it’s now 10:20 AM and nothing has hatched.  We are definitely past 144 hours post spawn…based on hatch times from prior clutches they should have hatched, but the 6+ day incubation time always seems short.  So hopefully I didn’t kill the eggs with the H202 treatment; there are no eggs on the ground, and nothing hatched, so hopefully it’s just another day and they’ll hatch tonight.  We shall see…

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