The Lightning Project

The ongoing saga of the PNG Lightning Maroon Clownfish Breeding Project

Browsing Posts tagged pre-spawn behavior

So this amounts to a chronological retelling of the story to date, this time with photos, starting  a couple weeks back now.  Perhaps not in as much detail as my minute-by-minute updates, but a good overview of the run to date.

June 21st, 2012

The ongoing health problems with the Lightning Maroon remained, and the left eye on the Lightning Maroon was showing slight swelling.

On a day initially planned to do a skin-scrape of the fish for further examination, I had to call things off because the fish had started going through pre-spawn motions.

By the time we had finished doing a skin scrape on some Banggai Cardinalfish downstairs, Barb & Heidi from the Great Lakes Aquarium got a super special treat, seeing the actual nest having been spawned while they were here.

Lightning Maroon Clownfish Spawning & Eggs

June 22nd, 2012

I was genuinely worried whether we’d have eggs 24 hours in.  Thankfully, they proved to be good parents and good “clownfish”; the first spawn egg eating proved to be the typical first test run that so many clownfish seem to do.  This batch, while I didn’t get a good photo of the parents, was doing well.  The swelling on the Lightning Maroon’s eye had gone away.  Phew.

June 23rd, 2012

So much for resting easy about the health of the Lightning Maroon. The eggs were developing (a fair number that probably were infertile or diseased were removed by the pair), but some funky gunk (yes, that’s the scientific term) showed up on the Lightning Maroon’s right face.  I was once again on high alert; this wasn’t pop-eye; this was more reminiscent of the mouth-rot I had to battle back a little while ago.

June 24th, 2012

So much for being on alert.  By evening, things looked so bad on the Lightning Maroon’s face that I pulled the trigger and initiated the third course of treatment with Maracyn SW and Maracyn II SW in this system.  The telltale bulge around the right eye had started to show as well.  I felt I had little other option at this point; this fish is simply too valuable to take a wait and see approach when symptoms like these show up:

The eggs were looking good and developing fast, although I took little comfort in that given the current situation with the Lightning Maroon.  The roller coaster of stress over this fish during the past couple months has been excruciating.  No doubt, there were times I pondered whether it would all be easier if the fish just passed away – of course solely a passing fancy, but when things are clearly out of your real control, it is incredibly tough to sit there and do “what you can”.  Of course, it’s a whole new level now that we are well within sight of the next major milestone in this 2+ year long project.

June 27th, 2012

June 27th represented the 4th day of Maracyn + Maracyn II treatments, and once again, it appeared I had potentially averted a crisis or loss.  The condition of the Lightning Maroon was drastically improved.  The eggs…the eggs were showing eyes?  They had the classic silvery look of clownfish eggs before they’re going to hatch.

I had been worried that these eggs would be hatching out while I was on a trip to Boston to speak at the Boston Reef Society; but now, only 6 days post spawn, I was very worried that a hatch could come sooner than expected.  The signs (and the data out there) said it was possible, sure, but maybe not likely?  Still, if I waited too long and did nothing I could miss the hatch. Conversely, if I pulled the nest too early, I could miss killing the eggs before they actually had fully developed.  Honestly though, I felt far less pressure about the decisions I was about to make than any of the disease-related issues with the Lightning Maroon; this is clownfish breeding, I can handle it.

There was really only one route to go – I had to sit and watch the tank.  The lights go off at 12:15 AM, so I got things situated for a possible hatch.  I used a small LED flashlight at the far corner of the tank as a larval attractant.

While waiting for the lights to go out, I prepared the area  with buckets and siphons to take out larvae should they hatch in the tank.

Downstairs, I prepared a black round tub to receive broodstock water and possible babies.

Lights went out, and it was time to wait.  All pumps were turned off through an extended feed timer on my Apex Lite (which would ensure they’d all come back on in the event that I somehow forgot about them and went to be).  I did have to unplug the battery backup on the Vortech…can’t have babies going through that pump either.  I’d check every once in a while, and initially got excited around 12:20 AM when I saw movement in the beam of the flashlight – until I realized it was copepods swimming around.

Many more checks turned up nothing, and I was starting to wonder if I had jumped the gun.  Multiple plans of “what next” rolled around in my head, but they all disappeared at 1:23 AM on June 28th, 2012.

That is not a copepod.  If you can’t really see it, maybe this one will help:

The moment that first baby clownfish showed up, I pulled the tile under almost complete darkness, moving it downstairs in a bucket with a lid and 5 gallons of water from the broodstock tank.  I set it up for artificial hatching, and assumed that come morning, I’d see hundreds of clownfish swimming around.  That was the hope…

June 28th, 2012

So much for hatching overnight.  There was ONE baby in the tub.  Terrific (<-sarcasm).  1 is better than none, so in the interest of keeping the one alive, I was forced to tinge the water green with a very light treatment of RotiGreen Nano, and a very small addition of rotifers (lest the baby starve).

The worst fear is that I had somehow killed the eggs in the move or prevented the hatch, which would have generally killed the eggs overnight.  There was only one way to find out.  I took a quick look at the tile.

And here’s what I saw…

They look perfectly fine.  And what a great opportunity, thanks to the advent of digital photography and Photoshop, to get a headcount.

That’s roughly 310 eggs (each color group represents me counting to 50, with the scattered red dots representing the last 10 I counted).  It’s not an exact headcount, but gives a great approximate number of eggs.  Hardly the spawn of several thousand that some Maroon Clownfish are known to put down, but I’ll take it all the same.  So very carefully, this tile went back into the black round tub…

…So long as the eggs didn’t die, there was still hope.  The rest of the tension filled day was spent fighting the urge to recheck the tile for dead eggs.  Come nightfall, I stuck with the photoperiod that the eggs had been used to, and turned the lights out in the basement a little early so that things were basically pitch black by 12:15 AM on June 29th.  Just after 1:00 AM, a quick check with the flashlight caused me to announce to the world, “Ladies and Gentleman; we’re rearing Lightning Maroon Larvae.

June 29th, 2012.

With only hours before my departure to Boston, I had to get things set up right.  As the night progessed into the wee hours of morning (that we normally still call “night”), I fired up the lights, and checked the tile:

No stragglers – that means a 100% hatch.  That means 300-ish baby maroon clownfish.  300 chances to see something really fantastic down the line.  So long as we don’t botch rearing them!

Mike Doty, a fellow aquarist who happens to live 4 blocks away from me, had been over late (or early if you want to get technical) to see how things were set up and to know where everthing was…well that and to share a beer, toasting this milestone. Mike would be completely in charge of rearing the larvae in my absence.

 

While I got my share of incredulous inquiries about that, I actually had more confidence in Mike than myself; Mike had taken a pair of extra Maroons from me, spawned and reared a couple batches, so he was perfectly qualified in my book (I’ve done clowns, but never maroons before).  We got the larval tub set up with greenwater and rotifers, and in the early afternoon I embarked on my all-day trek to Boston.

 

 July 1st, 2012

I returned home from Boston in the afternoon, anxious to see how things had gone.  Mike had kept me updated via texts during my absence and things sounded good.  The main message I got from Mike was that my three rotifer cultures had failed to keep up with demand, and he had actually depleted his as well.  I wondered, would we wind up losing this batch to starvation?!

July 2nd, 2012

I’m indeed burning through rotifers, but the cultures seemed to rebound and were producing enough for the moment.  The rotifers in the BRT were also clearing out phytoplankton pretty frequently.

Mike and I had set up a drip for the tub using a spare brine shrimp hatchery and a micro ball valve from Julian Sprung’s Two Little Fishies.  Not only is the drip good for top off, but also for introducing foods (phytoplankton) and ammonia control (CloramX) slowly.

Seeing that there were still many babies (some losses, but still many viable larvae), I took a photo for you all; your first look at what *Could be* a larval Lighting Maroon Clownfish, roughly 4 days old.

 July 5th, 2012

Things have gone well, as I’ve slowly doubled the larval rearing volume to 10 gallons, keeping a watchful eye on the ammonia alert badge as I continue to feed 4-5 gallons worth of rotifers into the tub per day.  With the warm basement temperatures (normally in the upper 60′s to lower 70′s, but lately 78F), the rotifer cultures are now roaring; I’m forced to feed them twice daily at a rate of 30 drops of RotiGrow Plus (and 30 drops CloramX).

I’ve done a couple pre-feeding rotifer enrichments with Super Selcon as well, just to keep the DHA levels up. However, today, now just before 7 days old, we reach another step in the rearing process.  Today it was decided the larvae were finally ready to feed on APBreed’s TDO, size A.  And after the second feeding, it was fair to say they are indeed consuming it.

So now we sit and wait.  Any day now, we will catch the first glimpses of stripes as these larval Maroon Clownfish go through metamorphosis and settle out into juveniles.  Most likely, I suspect that even if we have fish that will one day show the “Lightning” phenotype, we won’t see it at this stage in their development.  But at this time, it is anyone’s guess.  If you’re a betting man or woman, it’s time to place your wagers.  Our first glimpse at the possibilities are just around the corner.

This is the story of leaving home only to have the entire trip be a subconsciously gut wrenching experience. last week on the Tuesday before MACNA, Tony Vargas swung into town and stopped by with Boomer. Among other things, he sat glued to the Lightning Maroon’s tank for almost an hour, snapping dozens or hundreds of images. I can still remember Tony saying “I think I know where those fish are going to spawn.” pointing to the back wall of the tank where the pair was actively cleaning. Tony got several good shots, one of which is so good that none of you have seen it. I told Tony “that’s my book cover”. The below image is not the “uber good” one that he’s holding back..this was just a “good” one.

Lightning Maroon Clownfish photograph taken by Tony Vargas on 9-6-2011

I departed for MACNA 2011 (Des Moines, IA) on Thursday, leaving all my aquariums in top shape. They’d be able to withstand a bit of neglect and nothing should’ve gone wrong.

Of course, Friday evening I find I have two missed calls from my wife. I step outside and call her. She tells me that she came home and couldn’t find the Lightning Maroon, so she got worried. I too was getting worried but realized that now, on more than one occasion, this boldly patterned fish still manages to hide from view. It turned out that since calling, she found the fish. However, she hadn’t seen either clownfish eat. This again was cause for concern, so I had her check the APEX for both temperature and pH; the two parameters most likely to be out of whack (it wasn’t until SUNDAY, long after all this, that I even thought to ask her if all the pumps had been running…of course they had been the entire time). Everything was in order.

To me, this was both disconcerting and intriguing. Disconcerting in that for the fish to be off feed, something could be going terribly wrong – i.e. ammonia or nitrite levels rising. However, Renee doesn’t overfeed, and there was nothing big in the tank that could’ve died and caused a massive crash. So, if things are OK, when else have I not seen fish eating? Well, pretty much right before they’re spawning.

Saturday rolled around and I once again got new info. My friend Jay who breeds Maroon Clowns and sometimes stops by the house to watch the fish had come over, and he made the observation that the Lightning Maroon was going to be spawning. Renee said the fish were cleaning like crazy. Talking later with Jay, what he described almost sounded like pre-spawn runs, with the fish doing practice “touch and goes”. Or another way to think of it…mating without laying any eggs. The female would rub her belly down, then the male would follow. That sounds to me like spawning.

MACNA itself reminded me of home many times, with the Lightning Maroon showing up in my presentation, David Vosseler’s SEASMART discussions, and Julian Sprung’s talk too. I don’t remember names, but I know several people came up and asked if I was the guy with the Lightning Maroon. A bit surreal still.

Of course, I get home late Sunday, and a few of my friends at MACNA (most notably Kevin Erickson and Tony Vargas) have heard what is going on and are sharing my possible excitement. I made it home late, and to my relief everything appeared normally. I broke out a flashlight to look for eggs, but found none. Interestingly, both fish were sleeping together on the back tank corner, where I have seen a lot of cleaning activity occurring.

The final bit of excitement came last night, when I was paying close attention to the fish for a minute. I haven’t seen any cleaning activity since I returned home, but I did notice a slight extension coming off the Lightning Maroon’s belly on Monday night. Could this be an ovipositor? Could it in fact be that the Lightning Maroon pair DID spawn while I was away? Afterall, first clownfish clutches are often consumed by the parents.

Based on what I’ve heard and observed, I will go on record to say that I *think* we may have had our first spawn while I was away at MACNA. All of the reported information points to that, and the experiences of many clownfish breeders would support the guess. We will obviously never know (unless I have yet to locate a nest), but it’s possible that our first documented spawn could very well be coming sooner rather than later.

Weekend Update

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We’re seeing ongoing progress in the Lightning Maroon pairing.  More digging, more general surface cleaning, but no eggs yet.  Rod Buehler of Rod’s Food sent along an extra-special care package from Mama Percula to Mrs. Lightning this weekend…some of her secret recipe new “Breeder’s Blend”, aka. “No Candelight Needed”.  Some is getting tested out here, some has gone to another local breeder and we’re going to do an informal trial counting nest sizes to see how feeding this changes them over a few batches.

Rod's Food Breeder's Blend

There’s been some coral issues…I have a couple areas of tissue loss on a few of the smaller Birdsnest frags, and the Aussie Pinks, and Green, Gonioporas, have stopped extending their polyps.  A water test didn’t reveal anything causative…

pH (Seachem) – 8.2
pH (Apex) – 8.38
Total Alkalinity (Seachem) – 2.5 meq/L
Nitrate (Salifert) – 2 ppm
Calcium (Salifert) – 350 ppm
Magnesium (Salifert) – 1155 ppm

Based on the pH descrepency, I recalibrated the pH probe on the Apex Lite…afterwards it came in around 8.25 so we were definitely riding a bit high.  I’d been holding back on the C-Balance 2 part dosing due to elevated pH again, but turns out that probably wasn’t necessary.

Still, a 5 gallon (25%-ish) water change was ordered up.  We’ll see if that doesn’t help just a wee bit.

We are 110% on the right track. I know I alluded to the nest cleaning behavior here and other places, but today I caught it on camera. The Lightning Maroon was getting into it too, but the male PNG White Stripe Maroon Clown was doing the bulk of the substrate clearing. Of course, we know people who’ve had clownfish pairs do this behavior for months, or even years. Still, based solely on my clown pairs, you don’t see the female doing any of this unless she’s actually in the mood. I didn’t catch the Lighting Maroon doing any cleaning – I think my getting closer to the tank this evening kinda put “her” off. This is at least the third time I’ve seen them both going at it around the 6:00 PM hour…normally right when we’re having dinner. At any rate, enjoy the videos!

Forgive my wife’s laughing at the start of this one – our son was tasting lemonade for the first time and we’re pretty sure, based on the hysterical facial contortions he was making, it’s a love-hate relationship.

I took some time on Wednesday, July 27th, 2011, and Thursday (the 28th) to shoot these two videos that better show the behavior of this pair during daylight hours.  The first, from the 27th, was towards late evening when I find these fish rather retiring and shy – I had to use Nutramar Ova to get them more active.  The second was more mid-day on Thursday, a time at which the fish are more out and about.  ONE very interesting side note before I close with the two videos.  Thursday evening, while feeding our son dinner, I noticed both the PNG WS Male, and the Lightning Maroon, briefly engage in nest site cleaning behavior, nipping at the back wall of the tank.  Of course, that could mean anything.

On to the vids:

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