The Lightning Project

The ongoing saga of the PNG Lightning Maroon Clownfish Breeding Project

Browsing Posts tagged RotiGreen Omega

It’s the 7th night for Spawn #43 which was spawned on 10-22; we’re on the night of 10-29 going into 10-30 now; there wasn’t any noticeable hatch on the tile over the 6th night (I left it with the parents yesterady)….it will get pulled.  This is what Spawn #43 looked like when it was laid fresh on 10-22…I should also point out this is the first time I’ve taken pictures of the F0 original wild pair of Lightning Maroon X White Stripe PNG Maroon Clownfish.

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The larvae from spawn #42 that are still alive have largely settled out…it was a small group, but I’ll find a way to work with them.  More importantly, it looks like LM X LM Spawn #2 might be over…I have not found any larvae in the BRT, so one more look, I repurposed  it for Spawn #43.  The eggs got the usual dip in H2O2, but started hatching during it, so they were moved straight into the BRT with 8 gallons of broodstock water and a few ML of RotiGrow Plus (only thing I had thawed…it’ll be fine for the first 24 hours and by that point I’ll have RotiGreen Omega up and running).

The most frustrating news is arguably LM X LM Spawn #3.  So..to recap.  Here was the first spawn:

F1 (Lightning X Lightning) first spawn for the Lightning Maroon Clownfish

 

So…I took some proactive steps and tiled the back wall of the tank.

This was LM X LM (or LxL if you prefer) Spawn #2 on the first attempted hatch night:

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Yeah..that gray patch on the side..those are the eggs…

So that didn’t turn out obviously…so I tiled the side wall as well.

Here is Spawn #3, laid on 10/27/2014:

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The internet shorthand FML seems somehow appropriate. Clearly the pair is flipping me the middle fin.

 

As of 11 AM, it’s a small hatch on Spawn #34.  I added a little RotiGreen Omega to help keep the fish off the walls, and will check a bit later to see if the hatch has improved during the day.

I made a rare 8 PM trek down into the fishroom tonight (6/4/2014) and discovered the Lightning Maroon and her mate putting down Spawn #34. Based on how things are going, next Tuesday night should be the pull night.

My attempt to get a second night’s hatch out of Spawn #33 utterly failed. I did place the tile back with the parents come morning, and I went through the egg sanitizing protocol (with H2O2) for another 15 minute bath. I saw ONE larvae hatch out…a total dud.  My plan for the next spawn will be to pull the tile and 20 gallons out of the broodstock tank (BIG water change); 10 gallons each will go into two BRTs, and then I’ll simply move the tile from the first night’s hatch into the 2nd BRT for the 2nd night, sticking with the egg sanitizing protocol (as it seems to be helping?)

Those larvae that are alive from Spawn #33 are doing reasonably well..they are plowing through rotifers at an alarming rate; I’m adding in new saltwater along with RO/DI Water daily, as well as small feedings of TDO A to supplement for what I think might be a rotifer shortage. Low doses of ChloramX and RotiGreen Omega continue to be pulsed in as well.

It is now a fact – big Lightning Maroon Clownfish hatches are happening in the morning. I went to bed sometime after 4:30 AM on June 1st, and a few hours later, around 8:30, the kids woke me up. So, I went down to look at the BRT – just a handful of larvae.  I checked with a light, none on the bottom…just a very weak hatch.

I greened up the tub with RotiGreen Omega and added 2 gallon’s worth of rotifers from a culture. I turned on the light, left the tile in place.  Around 10:30 AM, I went down again and I now had hundreds of larvae in the BRT.

I pulled the tile and gave it back to the parents…can’t remember if that was an idea raised here, or on Facebook, but it seemed like a good way to go.  I have yet to go back down tonight, but I’ll presumably set up yet another new tank for a hatch tonight and see if we get two solid night’s worth of larvae off this batch.  Things could get interesting.

As near as I can tell, a lot of eggs likely hatched from the PNG White Stripe Maroon Clownfish pair’s 2nd spawn.  There are probably less than 50 eggs left.  Of course, this was a tiny nest to start with (compared to other Maroon nests) so I opted to just leave the few remaining eggs to fend for themselves tonight.  Last night would’ve been the “6th night” by my count, with tonight being the 7th night post spawn for these eggs.  I am hoping for a larger spawn and one that comes soon!

It’s just after 2 AM on May 6th, 2014, and I’ve prepared everything for an in-tank hatch with the Vossen Larval Snagger tonight.  By my count, this is the 6th night post hatch, the nest looks full and ready to go.  I took my own advice and set up the system’s pumps on a timer.  All water pumps shut off at 2 AM, and will turn on around 4 AM.  If this works well, I can simply move the 3-plug outlet (which runs internal filter, skimmer and internal UV) onto another outlet on the main power strip when not in use, but on hatch nights where I want to snag, I can just plug it into the timer (thus never having to reset the timer).

If I’m up still in an hour or so I’ll check the snagger to see what I caught so far…

Also ,the few larvae I snagged from Spawn #30 are going through settlement at this time; they have only been offered rotifers, no other feeds, with RotiGreen Omega for greenwater.  A  few have headstripes, and the rest should be getting near.

It’s been a busy January here, with my trip to Cleveland to give some well-received talks at C-SEA followed by a grueling week of fishroom preparation in advance of Reef Builder’s world-reknowned Jake Adams dropping by for a surprise visit and a bit of Q&A at the Lake Superior Marine Aquarium Club’s winter / holiday / new year bash. Of course, the Lightning Maroon breeding and rearing doesn’t stop because life gets in the way, but you better believe the online posts can sometimes drop in priority!

Spawn #20 has progressed, but not without hiccups. You may recall I split this batch between the 10 gallon tank and a 15 gallon BRT (black round tub), earlier this month. This proved to be a wise move. More on that in a second, but I found it extremely interesting to note that the larvae which were moved to the BRT under 24 hour light grew faster and underwent metamorphosis sooner…3-4 days sooner, than the ones left in the 10 gallon tank (which by default gets around an 8 hour dark period).  Just before my trip to Cleveland, I took this shot of the babies in the BRT:

Spawn #20 in the BRT, post metamorphosis, already easily discernable as white stripes or lightnings.

Spawn #20 in the BRT, post metamorphosis, already easily discernable as white stripes or lightnings.

During my time at C-SEA, my good friend and fellow clownfish breeder Mike Doty (you may recall he helped hatch and rear the very first Lightnings) was keeping an eye on the fishroom. For no reason, somewhere around the 17th or so, the babies in the 10 gallon just died. Mike can’t explain it, I can’t explain it. We’ve seen this happen before.

Meanwhile the ones in the BRT fared better, but there still have been losses. During one of Mike’s stop overs, he found 7 dead.  This photograph from 1-27-2014:

Lightning Maroon Clownfish Spawn #20 at 1/27/2014

Lightning Maroon Clownfish Spawn #20 at 1/27/2014

Most recently, on the 28th I started a water change, which normally is done with a very slow siphon into a 5 gallon bucket, the intake being placed so that it won’t drain the tank completely. Well…I didn’t have it really clamped down, and so it drained the BRT ompletely. Those babies which were still in a couple mm of water survived the 99.9% water change, but those that were in shallower water – damp, but not submerged – were dead. 21 lost totally due to a preventable accident. The upside is that the fish took a very traumatic, near 100% water change, and yet survived.

Victims of a water change gone awry.  Totally my fault.  Very frustrated over it, but moving on...

Victims of a water change gone awry. Totally my fault. Very frustrated over it, but moving on…

I’m guesstimating another 20-30 still alive. Either way, that shows you the losses through attrition that happen as these fish grow up – I stocked the BRT with 140 larvae.

Spawn #21 – I left Spawn #21 more or less in the hands of Mike to hatch…a handful of offspring had hatched out on the morning of the 17th (pulling the nest on the 16th was 7 days post spawn), and later that afternoon I had to depart for Cleveland. Unfortunately, the tile fell overnight, so come the 18th, instead of Mike finding a bunch of larvae hatched out, he came over to find a dead nest.

The few offspring that did make it from Spawn #21 underwent metamorphosis during Jake’s visit; it was pretty clear to see which were lightnings vs. not by Saturday night (the 25th)….this seems to be a pretty fast time to metamorphosis.  My headcount on offspring from this batch is somewhere around 4-6 post settlement…another very small run.  This photo also from 1/27/2014:

A couple of the survivors in Spawn #21, photographed 1/27/2014

A couple of the survivors in Spawn #21, photographed 1/27/2014

Spawn #22 – on the afternoon of 1/20/2014, the 22nd clutch of eggs was put down by the Lightning Maroon and her mate. Paying a close attention to things, I knew I could be pulling them as early as 6 days post spawn…so 21,22,23,24,24, evening of the 26th being 6 days on. Well, I took a gamble, left a little more ambient room light falling on the eggs, and found that on the morning of the 27th, we still had a nice, solid nest. Come the evening of the 27th, I pulled the tile along with 5 gallons of broodstock water and 5 gallons of new saltwater, and set them up with a wooden airstone incubation. I used a second tile to prevent the tile from falling, as well as to help weigh down and position the wooden airstone under the eggs. (I found my wooden airstones from eBay seller hoolko, who happened to be mentioned on Reef Builders a while back).

Spawn #22 set up and ready for hatching in a 10 gallon tank.

Spawn #22 set up and ready for hatching in a 10 gallon tank.  Note the larvae already hatched!

Within minutes of transferring the nest, I had a few larvae hatch, still in full light.

Newly hatched Lightning Maroon Clownfish offspring swims next to the Seachem Ammonia Alert Badge....

Newly hatched Lightning Maroon Clownfish offspring swims next to the Seachem Ammonia Alert Badge….

I left things go, not feeding or anything else. By the morning of January 28th, I had a few dozen larvae in the tank, but the bulk of the eggs remained unhatched. I weighed my options a bit, and ultimately decided to introduce some rotifers (about 2 gallons worth) but refrained from adding any phytoplantkon. I thought maybe I’d have more hatches later in the day, but come nightfall, nothing had happened. Would this be a botched hatch?

Apparently yes and no. This morning (the 29th) several hundred larvae were present in the tank, but many many more dead eggs were on the bottom. Most of the larvae held tightly to the black back wall of the 10 gallon tank.

Spawn #22 after the large hatch, before cleanup.

Spawn #22 after the large hatch, before cleanup.

I took the opportunity to first siphon off all the dead eggs (and dead larvae) on the bottom before tinting the tank with 50 drops of RotiGreen Omega.

Spawn #22 after cleaning up the larval tank.

Spawn #22 after cleaning up the larval tank.

I gave the rotifers their morning feeding of RotiGrow Plus, and later this evening they’ll get the next infusion of rotifers. I’m thinking I will once again work on a system of water changes, lowering salinity, and 24 hour lighting, to grow this batch, and as I discovered, I will once again at minimum split the batch early on.

The Holdback F1 Lightning Maroon Clownfish Pair

So I finally pulled the trigger on pairing up my holdbacks.  The Ecoxotic cube had been up and running for a while with a single holdback Lightning in it, so I swapped the fish and simply added in my Lightnings as a pair (they had formerly been neighbors, side by side) on 1/21/2014.

Initially, things went very well.  Here’s some video the day after, 1/22/2014.

Unfortunately, things didn’t continue down this blissful path.  A few days later, the larger fish turned on the smaller fish, damaging a few fins and forcing me to segregate the fish around 1/25/2014.  Currently, it is the larger, “future female” Lightning Maroon who resides in a drilled specimen cup.

Future pair of F1 Lightning Maroon Clownfish

Future pair of F1 Lightning Maroon Clownfish

The Holdback White Stripe Maroon F1 Pair

I should mention that somewhere in January I shuffled some fish around and introduced my two F1 PNG White Stripe holdbacks to each other as well.  The pairing has gone so-so.  They are not paired, but they continue to share their tank, the smaller fish cowering in a protected area but not otherwise excessively abused.  I’ll try to snag some photos at some point.  This will be a very important pairing to breed, as it will help definitively answer the question as to whether the “white stripe” siblings carry any special genetics (and it will prove or disprove the presence of a recessive Lightning gene).

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